DNA Synthesis | Genome Editing | Molecular Biology | Synthetic Biology | Synbio Technologies

1.Gene Information

*Species:

please specify:

Single gene, please fill in the Gene ID Genome, please upload your genome sequences

Gene ID:

Genome:

Please upload your genome sequences

2.sgRNA Design

Yes No

3-6 (Recommended) Other,please specify:

Please upload your sgRNA sequences

3.sgRNA Synthesis

Ready-to-use sgRNA (in vitro transcription) Cloning into vector

Amount:

please specify

*Vector name:

*Vector size: bp

*Resistance:

*Copy number: High Low

Commercial vector: Yes No

Vector sequence:

5' cloning site:

3' cloning site:

Cas 9: Synbio Technologies’ recommendation Other,please specify:

Ship the vector to Synbio Technologies:

Vector map (optional):

4.Deliverables

Standard delivery: 2-5 µg lyophilized plasmid Custom plasmid preparation (extra charge) sgRNA Library: 2-5 µg liquid plasmid In vitro transcription RNA: 10-15ug liquid RNA Others

Please specify:

Custom plasmid preparation :

Quality grade:
Research Grade (Endotoxin, N/A) Transfection Grade (Endotoxin < 100EU/mg)

Desired buffer:

5.Lentivirus Package

No Yes

*Titer:

*Amount:

Negative Control: Yes No

6.Stable cell line construction

No Yes

*Cell Type:

Other Requirements

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Step 1: Project Design